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vector phrdsv40 scfv gcn4 sfgfp vp64 gb1 nls  (Addgene inc)


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    Structured Review

    Addgene inc vector phrdsv40 scfv gcn4 sfgfp vp64 gb1 nls
    Vector Phrdsv40 Scfv Gcn4 Sfgfp Vp64 Gb1 Nls, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+60904/pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS+(Plasmid+%2360904)/bio_rxiv__64898__2026__01__14__699605-236-15-17
    Average 95 stars, based on 82 article reviews
    vector phrdsv40 scfv gcn4 sfgfp vp64 gb1 nls - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Amplification:

    Article Title: DNA methylation editing kit and DNA methylation editing method
    Article Snippet: A GFP fragment was amplified from Addgene plasmid 60904. .. An ScFv fragment was also amplified from Addgene plasmid 60904. ..

    Article Title: DNA methylation editing kit and DNA methylation editing method
    Article Snippet: .. A GFP fragment was amplified from Addgene plasmid 60904. .. An ScFv fragment was also amplified from Addgene plasmid 60904.

    Article Title: BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity
    Article Snippet: .. The scFv and GB1 fragments were amplified from Addgene plasmid 60904 by PCR amplification. .. The APOBEC and UGI fragments were amplified from Addgene plasmid 73021 by PCR amplification.

    Plasmid Preparation:

    Article Title: DNA methylation editing kit and DNA methylation editing method
    Article Snippet: A GFP fragment was amplified from Addgene plasmid 60904. .. An ScFv fragment was also amplified from Addgene plasmid 60904. ..

    Article Title: DNA methylation editing kit and DNA methylation editing method
    Article Snippet: .. DNA encoding the anti-tag peptide antibody can be obtained by a known molecular biological technique, can be obtained by amplifying, for example, a commercially available plasmid such as Addgene plasmid 60904 by PCR, or may be artificially produced using an artificial gene synthesis technology known to those skilled in the art, and a method of obtaining the DNA is not limited. .. The anti-tag peptide antibody or RNA encoding the anti-tag peptide antibody can be obtained by inserting the DNA encoding the anti-tag peptide antibody into an appropriate expression vector and expressing the protein or the RNA. (Methylase and Demethylase) The methylase in the present is can be used without limitation as long as being an enzyme that catalyzes the methylation of an unmethylated site, and includes a methylase which is an enzyme that methylates a particular base on a DNA nucleotide sequence, and a methyltransferase which is an enzyme transferring a methyl group to a particular base, and more specific examples thereof include DNA methyltransferase 3 beta (DNMT3B), DNA methyltransferase 3 alpha (DNMT3A), and DNA methyltransferase 1 (DNMT1).

    Article Title: DNA methylation editing kit and DNA methylation editing method
    Article Snippet: .. A GFP fragment was amplified from Addgene plasmid 60904. .. An ScFv fragment was also amplified from Addgene plasmid 60904.

    Article Title: BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity
    Article Snippet: .. The scFv and GB1 fragments were amplified from Addgene plasmid 60904 by PCR amplification. .. The APOBEC and UGI fragments were amplified from Addgene plasmid 73021 by PCR amplification.

    Polymerase Chain Reaction:

    Article Title: DNA methylation editing kit and DNA methylation editing method
    Article Snippet: .. DNA encoding the anti-tag peptide antibody can be obtained by a known molecular biological technique, can be obtained by amplifying, for example, a commercially available plasmid such as Addgene plasmid 60904 by PCR, or may be artificially produced using an artificial gene synthesis technology known to those skilled in the art, and a method of obtaining the DNA is not limited. .. The anti-tag peptide antibody or RNA encoding the anti-tag peptide antibody can be obtained by inserting the DNA encoding the anti-tag peptide antibody into an appropriate expression vector and expressing the protein or the RNA. (Methylase and Demethylase) The methylase in the present is can be used without limitation as long as being an enzyme that catalyzes the methylation of an unmethylated site, and includes a methylase which is an enzyme that methylates a particular base on a DNA nucleotide sequence, and a methyltransferase which is an enzyme transferring a methyl group to a particular base, and more specific examples thereof include DNA methyltransferase 3 beta (DNMT3B), DNA methyltransferase 3 alpha (DNMT3A), and DNA methyltransferase 1 (DNMT1).

    Article Title: BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity
    Article Snippet: .. The scFv and GB1 fragments were amplified from Addgene plasmid 60904 by PCR amplification. .. The APOBEC and UGI fragments were amplified from Addgene plasmid 73021 by PCR amplification.

    Produced:

    Article Title: DNA methylation editing kit and DNA methylation editing method
    Article Snippet: .. DNA encoding the anti-tag peptide antibody can be obtained by a known molecular biological technique, can be obtained by amplifying, for example, a commercially available plasmid such as Addgene plasmid 60904 by PCR, or may be artificially produced using an artificial gene synthesis technology known to those skilled in the art, and a method of obtaining the DNA is not limited. .. The anti-tag peptide antibody or RNA encoding the anti-tag peptide antibody can be obtained by inserting the DNA encoding the anti-tag peptide antibody into an appropriate expression vector and expressing the protein or the RNA. (Methylase and Demethylase) The methylase in the present is can be used without limitation as long as being an enzyme that catalyzes the methylation of an unmethylated site, and includes a methylase which is an enzyme that methylates a particular base on a DNA nucleotide sequence, and a methyltransferase which is an enzyme transferring a methyl group to a particular base, and more specific examples thereof include DNA methyltransferase 3 beta (DNMT3B), DNA methyltransferase 3 alpha (DNMT3A), and DNA methyltransferase 1 (DNMT1).



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